Regulatory

Part:BBa_K3347017:Design

Designed by: Jackson Duke Harris   Group: iGEM20_USAFA   (2020-10-16)


Improved prma promoter from Rhodococcus jostii RHA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 150
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence of prma was identified in the genome of RHA1 using the known genome from NCBI. Primers were designed to amplify the promoter containing region and incorporate EcoR1, Xba1, Spe1, and Pst1 sites so that standard iGEM assembly could be performed.


Source

Original sequence: BBa_K2911000

References